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NEP Monoclonal Antibody Production

NEP Monoclonal Antibody Production

To satisfy our customers expanding needs and complement our sterling reputation as a supplier of top notch custom polyclonal antibodies, New England Peptide, Inc. also offers custom monoclonal antibody production.

This service is broken down into the typical multi-phase development strategy for flexibility purposes.

Phase I: Inception                    $4,000

• NEP will analyze target protein(s) and make peptide for immunization and screening
• Additional screening methods will be discussed and finalized (additional charges may apply)
• Three mice (Balb/c) will be immunized over a period of seven weeks
• Anti-sera will be analyzed by ELISA using appropriate material and/or samples sent to customer for evaluation

Phase II: Development             $6,000

• Splenocyte cells from top mouse will be fused with a myeloma cell line
• Fusions will be screened for hybridomas producing specific antibodies by ELISA
• Expanded fusion positives will be screened in a reconfirmation assay from which up to twenty different parental clones will be selected and supernatant samples sent to customer for evaluation
• One vial of each parental clone will be frozen and stored for future use 

Phase III: Sub-cloning              $1,250 each

• Sub-cloning of parental cell line with ELISA screening and expansion
• Up to two positive sub-clones from each parental clone will be expanded and isotyped, with a final deliverable of 10mL supernatant from each sent to the customer for evaluation
• All remaining material is frozen and can be banked for a period of one year

Phase IV: Production                To Scale

• Scale-up production and purification

 

get custom monoclonal antibodies quote

Or contact us at antibody@newenglandpeptide.com.  As always do not hesitate to call us at 888-343-5974.

Testimonials

"We have no reason to use any other antibody company at this point! We are very pleased with your work. We realize the difficulty in obtaining high quality phosphorylated antibodies so we desire to stick with NEP. Thank you for your work on this!"
Beth A. PhD - Florida State University