The Product Spotlight – New Offerings, Including COVID-Items, By the Numbers

As happens occasionally, New England Peptide and Peptides International find ourselves with a large number of new releases from our own labs as well as from our partners.  We will begin with items relating to the COVID-19 coronavirus.   

Our growing list of coronavirus items has seven new additions.  Five of these are recombinant proteins, related to the COVID-19, including segments and spike proteins.  The sixth is a peptide fragment of the putative orf1ab polyprotein [SARS coronavirus] and the seventh, a serine protease inhibitor has other interesting applications as well. We would also like you alert you to our brand new page: The COVID-19 Information Station, designed to be an aggregator of all our related COVID-19 info.

Some background* - A human infecting coronavirus (viral pneumonia) called 2019 novel coronavirus, 2019-nCoV was found in the city of Wuhan, Hubei province of China on December 2019.

The 2019-nCoV shares an 87% identity to the 2 bat-derived severe acute respiratory syndrome 2018 SARS-CoV-2 located in Zhoushan of eastern China. 2019-nCoV has an analogous receptor-BD-structure to that of 2018 SARS-CoV, even though there is a.a. diversity, thus the 2019-nCoV might bind to ACE2 receptor protein (angiotensin-converting enzyme 2) in humans.

RSCU analysis proposes that the 2019-nCoV is a recombinant within the viral spike glycoprotein between the bat coronavirus and an unknown coronavirus.

  • nCoV-S1
    SARS-011
    Novel Coronavirus 2019-nCoV Spike Glycoprotein-S1, Recombinant
  • nCoV-S2
    SARS-012
    Novel Coronavirus 2019-nCoV Spike Glycoprotein-S2, Recombinant
  • CoV-2019 Nucleocapsid
    SARS-013
    Coronavirus 2019 Nucleocapsid Recombinant
  • CoV Spike E-Mosaic
    SARS-014
    Coronavirus 2019 Spike E-Mosaic Recombinant

  • CoV N-Mosaic
    SARS-015
    Coronavirus 2019 Nucleocapsid Mosaic Recombinant
  • Putative orf1ab Polyprotein (3233-3247) [SARS coronavirus]
    BP12-331
    H2N-SITSAVLQSGFRKMA-OH

*background content supplied by ProSpec

Camostat mesilate is a serine protease inhibitor that inhibits plasmin, kallikrein, thrombin as well as trypsin, which attenuates pancreatic fibrosis.  It reduces weight gain and improves metabolism in obese rodent models.  It is in clinical use (in Japan) for pancreatitis.  Camostat has been found to inhibit influenza virus replication in human tracheal epithelial cells and is also a direct prostasin inhibitor which may be useful in reducing sodium transport in cystic fibrosis. Additionally, it has been shown to reduce infection of Calu-3 lung cells by SARS-CoV-2 (the coronavirus responsible for COVID-19) via inhibition of the serine protease TMPRSS2 required for viral spike protein priming.

https://www.pepnet.com/res/uploads/media/Camostat-mesilate-ISP-3606-PI-.jpg

  • Camostat Mesilate
    ISP-3606-PI
    4-(2-(2-(Dimethylamino)-2-Oxoethoxy)-2-Oxoethyl)Phenyl 4-Guanidinobenzoate, Methanesulfonic Acid Salt; FOY-305; Foipan
    (M.W. 494.5) C20H22N4O5 • CH3SO3H     [59721-29-8]
    A Serine Protease Inhibitor; Shown to Inhibit Viral Entry Into Human Lung Cells

    J Gibo, et al., Lab. Invest., 85, 75 (2005).
    K Albarazanji, et al., Am. J. Physiol. Gastrointest. Liver Physiol., 316, G653 (2019).
    M Ueda, et al., Nephron, 129, 223 (2015).
    M Yamaya, et al., Pulm. Pharmacol. Ther., 33, 66 (2015).
    M Hoffmann, et al., Cell, 181, 1 (2020).
    S. M. Rowe, et al., Chest., 144(1), 200 (2013).


While there is no denying the disruptions from and research interest about the current pandemic, we have other compelling items worth sharing too.

Another serine inhibitor, AEBSF • HCl, is a broad spectrum, water soluble, and irreversible inhibitor of serine proteases, targeting chymotrypsin, kallikrein, plasmin, trypsin, and thrombin.

 

  • AEBSF • HCl
    ISP-3604-PI
    4-(2-Aminoethyl)-Benzene Sulfonylfluoride • HCl
    (M.W.239.69) C8H10FNO2S∙HCl.    [30827-99-7]
    Protease Inhibitor

 There are four new cyclic dipeptides, or diketopiperazines, also added this month.

  • Cyclo(Ala-Pro) (Bulk)
    BCD-3244
    Cyclo(Pro-Ala), Cyclo(-Ala-Pro-)
    (M.W. 168.19) C8H12N2O2     [36357-32-1]
  • Cyclo(Lys-Pro) (Bulk)
    BCD-3245
    Cyclo(Pro-Lys), Cyclo(-Lysa-Pro-)
    (M.W. 225.29) C11H19N3O2     [111688-85-8]
  • Cyclo(Pro-Pro) (Bulk)
    BCD-3246
    Cyclo(Pro-Pro), Cyclo(-Pro-Pro-)
    (M.W. 194.23) C10H14N2O2     [19943-27-2]

  • Cyclo(Thr-Pro) (Bulk)
    BCD-3247
    Cyclo(Thr-Ala), Cyclo(-Thr-Pro-)
    (M.W. 198.22) C9H14N2O3     [227777-31-3]

There is a veritable bestiary represented with our peptide toxins, but recently plants have an increasing representation in the catalog.  This one is an antimicrobial derived from the potato.

  • Snakin-1 (0.1mg vial)
    AMP-4503-s
    SN1, GSL1, Gly-Ser-Asn-Phe-Cys-Asp-Ser-Lys-Cys-Lys-Leu-Arg-Cys-Ser-Lys-Ala-Gly-Leu-Ala-Asp-Arg-Cys-Leu-Lys-Tyr-Cys-Gly-Ile-Cys-Cys-Glu-Glu-Cys-Lys-Cys-Val-Pro-Ser-Gly-Thr-Tyr-Gly-Asn-Lys-His-Glu-Cys-Pro-Cys-Tyr-Arg-Asp-Lys-Lys-Asn-Ser-Lys-Gly-Lys-Ser-Lys-Cys-Pro
    Antimicrobial Peptide
    Solanum tuberosum
    Disulfide bonds between Cys5-Cys30, Cys9-Cys26, Cys13-Cys22, Cys29-Cys62, Cys33-Cys49 and Cys35-Cys47
    1427832-11-8

Segura, M. Moreno, F. Madueno, A. Molina, and F. Garcia-Olmedo, Mol. Plant-Microbe Interact., 12, 16 (1999). (Original)
P.W.R. Harris, S.-H. Yang, A. Molina, G. Lopez, M. Middleditch, and M.A. Brimble, Chem. Eur. J., 20, 5102 (2014). (Chem. Synthesis)
H. Yeung, C.J. Squire, Y. Yosaatmadja, S. Panjikar, G. Lopez, A. Molina, E.N. Baker, P.W. Harris, and M.A. Brimble, Angew. Chem. Int. Ed., 55, 7930 (2016). (S-S Bond)
M.R. Kuddus, F. Rumi, M. Tsutsumi, R. Takahashi, M. Yamano, M. Kamiya, T. Kikukawa, M. Demura, and T. Aizawa, Protein Expr. Purif., 122, 15 (2016). (Rec. Synthesis)
H. Senechal, J. Santrucek, M. Melcova, P. Svoboda, J. Zidkova, D. Charpin, L. Guilloux, Y. Shahali, M.A. Selva, R. Couderc, T. Aizawa, and P. Poncet, J. Allergy Clin. Immunol., 141, 411 (2018). (Pharmacol.)
T. Wu, et al., Citrus. Plant Growth Regul. (2020). https://doi.org/10.1007/s10725-020-00589-1

Finally, there are five peptide tools you may want to consider, all stable isotope-labeled:

  • Val-His-[D7]Leu-Thr-Pro-Glu (50 μg vial)
    Trifluoroacetate Salt
    TSI-3414-v
    Val-His-*Leu-Thr-Pro-Glu
    *Leu: Leucine-(isopropyl-d7)
    (M.W. 701.82) C31H43D7N8O10
    Stable Isotope-Labeled N-Terminal Hexapeptide of Hb β-Chain

U. Kobold, J.O. Jeppsson, T. Duelffer, A. Finke, W. Hoelzel, and K. Miedema, Clin. Chem., 43, 1944 (1997).
T. Nakanishi, K. Iguchi, and A. Shimizu, Clin. Chem., 49, 829 (2003). (Measurement Using The Isotope-labeled Compound)

  • 1-Deoxyfructosyl-Val-His-[D7]Leu-Thr-Pro-Glu (50 μg vial)
    Trifluoroacetate Salt
    TSI-3415-v
    1-Deoxyfructosyl-Val-His-*Leu-Thr-Pro-Glu
    *Leu: Leucine-(isopropyl-d7)
    (M.W. 863.96) C37H53D7N8O15
    Stable Isotope-Labeled N-Terminal Hexapeptide of HbA1c β-Chain

U. Kobold, J.O. Jeppsson, T. Duelffer, A. Finke, W. Hoelzel, and K. Miedema, Clin. Chem., 43, 1944 (1997).
T. Nakanishi, K. Iguchi, and A. Shimizu, Clin. Chem., 49, 829 (2003). (Measurement Using The Isotope-labeled Compound)

  • 4-[D10]Leu-M1 (20 μg vial)
    Trifluoroacetate Salt
    TSI-3421-v
    [2H10]LeuB6,B11,B15,B17]-M1
    A-chain: Gly-Ile-Val-Glu-Gln-Cys-Cys-Thr-Ser-Ile-Cys-Ser-Leu-Tyr-Gln-Leu-Glu-Asn-Tyr-Cys-Gly
    B-chain: Phe-Val-Asn-Gln-His-[2H10]Leu-Cys-Gly-Ser-His-[2H10]Leu-Val-Glu-Ala-[2H10]Leu-Tyr-[2H10]Leu-Val-Cys-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Thr
    ( Disulfide Bonds Between CysA6-CysA11, CysA7-CysB7, CysA20-CysB19)
    (M.W. 5790.8) C255H340D40N64O76S6
    Stable Isotope-Labeled Insulin Glargine Metabolite M1

Y. Xu, L. Sun, M. Anderson, P. Belanger, V. Trinh, P. Lavallee, B. Kantesaria, M.J. Marcoux, S. Breidinger, K.P. Bateman, D. Goykhman, and E.J. Woolf, J. Chromatogr. B, 1063, 50 (2017).

  • 6-[D10]Leu-Glargine (20 μg vial)
    Trifluoroacetate Salt
    TSI-3420-v
    [[2H10]LeuA13,A16,B6,B11,B15,B17]-Glargine
    A-chain: Gly-Ile-Val-Glu-Gln-Cys-Cys-Thr-Ser-Ile-Cys-Ser-[2H10]Leu-Tyr-Gln-[2H10]Leu-Glu-Asn-Tyr-Cys-Gly
    B-chain: Phe-Val-Asn-Gln-His-[2H10]Leu-Cys-Gly-Ser-His-[2H10]Leu-Val-Glu-Ala-[2H10]Leu-Tyr-[2H10]Leu-Val-Cys-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Thr-Arg-Arg
    (M.W. 6123.3) C267H344D60N72O78S6
    Stable Isotope-Labeled Insulin Glargine

Y. Xu, L. Sun, M. Anderson, P. Belanger, V. Trinh, P. Lavallee, B. Kantesaria, M.J. Marcoux, S. Breidinger, K.P. Bateman, D. Goykhman, and E.J. Woolf, J. Chromatogr. B, 1063, 50 (2017).

  • Val-His-Leu-Thr-Pro-Glu (0.5 mg vial)
    Trifluoroacetate Salt
    TSI-3413-v
    L-Valyl-L-Histidyl-L-Leucyl-L-Threonyl-L-Prolyl-L-Glutamic Acid
    (M.W. 694.78) C31H50N8O10
    N-Terminal Hexapeptide of Hb β-Chain

 Of course, if you need a another heavy-labeled peptide for proteomics or mass spectroscopy applications, we can provide custom NEPTune™ and TrypTides™ (tryptic peptides).

Testimonials

"The phospho-NL-4 Ab is fantastic, so that is great news! We have a VERY exciting study ongoing with it.
Not sure if you saw but the original phospho NL-1 Ab you guys made for us we just published in Nature Neuroscience. Thanks for making great antibodies, and we look forward to keep working with you guys!"
Michael B. PhD – NIH