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Care and Storage of Antibodies

Serum

For short term storage, store at 4°C. For long term storage (longer than one month), add 0.05% azide and store at -20°C. Serum is stable at -20°C for two to three years.

Purified Antibody (supplied in PBS, pH 7.4)

For best results, store at -80°C. If a -80°C freezer is not available, any combination of adding 0.05% azide, 1% BSA, and 50% glycerol will help stabilize the antibody when stored at -20°C. Antibody is stable at -80°C (or with 50% glycerol at -20°C) for two to three years.

If storing material at -80°C, we also recommend that you determine a working amount of antibody and subsequently apportion the bulk into single-use aliquots to prevent damage caused by freeze-thaw cycles.

Canadian customers, please note:

Due to the difficulties in sending material over the border on dry ice, we have already added 50% glycerol to your antibody. Store at -20°C.

Gold Conjugated Purified Antibody (supplied in PBS, pH 8.2)

For short term storage, room temperature is perfectly acceptable. For long term storage, store at 4°C.  Do not freeze unless at least 20% glycerol has been added!

 

Restoration of Lyophilized Material

Serum:

Add dH2O to equal the amount written on the label. Store at -20°C for best results.

Purified Antibody:

Divide the total amount of antibody by the concentration to determine the original volume and add dH2O in that amount. For generation of a specific concentration you can bring up the antibody in a different amount of dH2O (i.e., 5mg in 5mL for 1.0mg/mL) if you prefer. For best results, store restored material at -80°C, or add 30% glycerol and store at -20°C.

Testimonials

"We have no reason to use any other antibody company at this point! We are very pleased with your work. We realize the difficulty in obtaining high quality phosphorylated antibodies so we desire to stick with NEP. Thank you for your work on this!"
Beth A. PhD - Florida State University