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Custom Peptide Arrays

Custom Peptide Arrays

NEP's proprietary peptide array platform offers customers a rapid, cost-effective way to synthesize 96 different peptides. Unique to New England Peptide, Flashpure™ Custom Peptide Arrays are purified using NEP's proprietary reverse phase purification process to remove all non-peptide contaminants. These contaminants can result in false negatives and positives as well as poor peptide solubility. Flashpure™ is included in all arrays at no additional cost. Array peptides are unbound and delivered in 96 individual tubes, in a 96-well plate format. Each plate is individually tested for accuracy and can be used for epitope mapping, peptide libraries, protein characterization and much more. We offer up to 20-mer peptides at 2.5μm scale. We also offer 3 tiers of analysis and delivery is only 2-3 weeks. Using this format, peptides cost as little as $22 each!

    • 96 FlashPure™ peptides per plate in 96 individual tubes in 96-well plate format
    • 1-2mg average for a 15 amino acid peptide (individual weights per tube not provided)
    • No cross contamination
    • 2-3 week delivery
    • QC of each individual plate (analysis tiers listed below)
    • Biotinylation, Fluorescein, Stable Isotope Labeling and other modifications available
    • The array is best suited for peptides that are 12-18 amino acids in length. Max length is 24 amino acids
    • The average purity is of a 15-mer is ~75%. The approximate failure rate per plate is <5%. Failure rate may increase with longer peptides, hydrophobic sequences, and modified sequences

 
Three Analysis Options (per plate cost):

Tier 1 - $2,475

Mass Spec of 5 peptides

Tier 2 - $3,300

Mass Spec of every peptide

Tier 3 - $5,900

Mass Spec and HPLC of every peptide

 

Partial plates are available with a minimum of 24 peptides.  

 

NEPTune™ Discovery Array

Heavy/light tryptic peptide fragments for MRM and Quantitative Proteomics.

    • 96 FlashPure™ peptides per plate in 96 individual tubes in 96-well plate format
    • 1.0 μmol scale (~0.5 - 1.0 mg average for a 15 amino acid peptide)
    • Heavy Arg (+10) or Lys (+8) at C-terminus
    • CAM cysteine protection
    • Other PTM's including phospho residues (Ser, Thr, Tyr)
    • Optimized for sequences up to 15 amino acids in length, average 75% purity
    • No cross contamination
    • 2-3 week delivery
    • QC of each individual plate (analysis tiers listed above)
    • The array is best suited for peptides that are 12-18 amino acids in length. Max length is 24 amino acids
    • The average purity is ~75%.

 
To request an Array quote, please contact us at sales@newenglandpeptide.com .  Always make sure to include peptide sequences along with any modifications required.  Or call us at 888-343-5974.
 

Please note: Peptide Arrays are for High Throughput Screening, and peptide purities may vary from 50-95%. The array is best suited for peptides that are 12-18 amino acids in length. The approximate failure rate per plate is <5%. Failure rate may increase with longer peptides (max length is 24 amino acids), hydrophobic sequences and modified sequences.  NEP does not guarantee the purity or success of any one peptide on an array plate.

 

Just a Few of the Many References for New England Peptide Arrays:

(1)   Protective immunity to ricin toxin conferred by antibodies against the toxin's binding subunit (RTB). Yermakova, A., and Mantis, N. J. (2011) Vaccine 29, 7925-7935 http://www.sciencedirect.com/science/article/pii/S0264410X1101334X

(2)   Detailed Characterization of T Cell Responses to Herpes Simplex Virus-2 in Immune Seronegative Persons. Posavad, C. M., Remington, M., Mueller, D. E., Zhao, L., Magaret, A. S., Wald, A., and Corey, L. (2010) The Journal of Immunology 184, 3250-3259 http://jimmunol.org/content/184/6/3250.abstract

(3)   Expanded Breadth of the T-Cell Response to Mosaic Human Immunodeficiency Virus Type 1 Envelope DNA Vaccination. Kong, W.-P., Wu, L., Wallstrom, T. C., Fischer, W., Yang, Z.-Y., Ko, S.-Y., Letvin, N. L., Haynes, B. F., Hahn, B. H., Korber, B., and Nabel, G. J. (2009) J. Virol. 83, 2201-2215 http://jvi.asm.org/cgi/content/abstract/83/5/2201

(4)   Development and application of monoclonal antibodies against avian influenza virus nucleoprotein. Yang, M., Berhane, Y., Salo, T., Li, M., Hole, K., and Clavijo, A. (2008) Journal of Virological Methods 147, 265-274 http://www.sciencedirect.com/science/article/pii/S0166093407003655

(5)   Targeting of humanized antibody D93 to sites of angiogenesis and tumor growth by binding to multiple epitopes on denatured collagens. Freimark, B., Clark, D., Pernasetti, F., Nickel, J., Myszka, D., Baeuerle, P. A., and Van Epps, D. (2007) Molecular Immunology 44, 3741-3750 http://www.sciencedirect.com/science/article/pii/S0161589007001368

(6)   Kinetochore Microtubule Dynamics and Attachment Stability Are Regulated by Hec1. DeLuca, J. G., Gall, W. E., Ciferri, C., Cimini, D., Musacchio, A., and Salmon, E. D. (2006) Cell 127, 969-982 http://www.sciencedirect.com/science/article/pii/S0092867406014085

Testimonials

"Over the past 8 years we have used NEP for synthesis of many hundreds of peptides. The quality of NEP’s product is consistently very high. The company is deeply knowledgeable in all aspects of peptide synthesis, highly collaborative, and keenly interested in meeting the changing needs of their customers with respect to scale and formatting for delivery of peptides. One example is their flash-purified product which meets the need for initial draft-level evaluation without breaking the bank. Our experience with NEP’s antibody production capabilities has also been very good..."
Steven Carr, PhD - Director of Proteomics, Broad Institute