'I have tried many peptide providers for proteomics applications, but NEP is the only peptide producer that has met or exceeded our expectations with every single order. Thank you for your focus on developing and delivering solutions for the proteomics community.'

NEP Customer

Peptides for Quantitative Proteomics (PQP)

Revolutionize your quantitative proteomics projects with isotopically labeled peptide standards from NEP.  NEP’s PQP suite of products and services is designed to eliminate the frustrations of the  proteomics scientist. Our solutions enable you to focus on the next breakthrough in biomarker discovery. NEP’s services are enabling researchers around the world to develop the next generation of molecular diagnostics for applications such as personalized medicine and beyond.

Experience Reproducibility

You have found your source for consistent results. We recognize that protein identification and quantitation are compared across laboratories and platforms around the globe. We understand that success depends on consistent results that transcend the testing venue. Until NEP introduced its PQP platform, the proteomics community didn’t know where to turn for these services. As the proven leader in peptide quality, NEP was the right firm to answer the call for reproducibility.

PQP Value Chain

NEP took a holistic approach to developing its mix of PQP services. From starting material to storage to support, our soup-to-nuts offering is tailor made to fit the needs of those engaged in biomarker discovery.

Starting Materials

We are firm believers in the adage “you get out what you put in.” We understand that the quality of starting material in biomarker discovery is of paramount importance. That’s why we have supply partnerships with the top providers of the key starting materials required in proteomics research. Of particular importance:

• Isotopically labeled amino acids are not all created equal. Not every amino acid vendor can achieve the isotopic purity required to attain your desired results which is why we only work with the best.
• Security AND timeliness of supply. We know there is nothing worse than placing an order and being told there will be a delay as your provider awaits delivery of the raw material before peptide production can even begin.

Production

• Optimized chemistries for C-terminally isotopically-labeled peptides (ILP)
• Custom synthesis and site-specific incorporation of isotopically-labeled residues for researcher-driven PQP applications
• Matched sets of labeled and non-labeled peptides for enhanced validation and quantitation
• Production at scales aligned with the needs of the proteomics community
• Quality controls that ensure reproducible results

Project Management

Tasked with keeping each customer apprised of the progress of their order, NEP’s project management team is comprised of people who have spent the bulk of their careers in the laboratory producing peptides. This experience enables them to deftly handle technical questions and provide informative and timely project updates so you can focus on your work, not your peptide vendor’s. 

Analysis

NEP understands that analytical tests must be highly sensitive and accurate. We appreciate that while some applications may tolerate some variance in analyses, proteomics research requires exquisite consistency. Errors in data analysis can invalidate thousands of samples and weeks or months of research. Our analytical services are designed with the proteomics researcher in mind so every assay counts.

• HPLC to define chemical purity of the peptide. 
• MALDI-MS to provide confirmation of identity and isotopic incorporation to give desired mass shift.
• Amino Acid Analysis (AAA) validated against National Institute of Standards and Technology (NIST) protein standards. Used to define absolute peptide content or concentration of samples or aliquots
• Additional analytical services tailored to the needs of the PQP community including:

o        LC-MS and MS/MS Fragmentation

o        High resolution MS to confirm isotopic incorporation and pattern of peptide

Sample Preparation

We recognize sample preparation is critical to achieving consistent, dependable results.

Storage options

Aliquots

• Aliquots down to picomole scale
• Proprietary automated aliquotting system

Vials

• Customer choice of vial
• Customized labels, including color coding
• Bar coding

Racks

• Can be set up to integrate with robotic sample handling platforms

Scientific Support

Our commitment to our customers doesn’t stop when we ship your products. NEP’s team is available to field any questions that arise during or between projects.

Have questions or need a quote? Call 888-343-5974 or email proteomics@nepeptide.com.

Why NEP for Proteomics

Single Source

NEP is the market’s first single source for all of your peptide proteomics needs. Why waste time cobbling together solutions from multiple vendors, when you can leave it to NEP and return to your research?

People

NEP’s team is comprised of the most accomplished people in peptide production and proteomics research yet we haven’t lost sight of the details and processes required to deliver your PQP products quickly and in a format best suited for your needs. From our day-to-day scientific leadership to our scientific advisory board, you get unparalleled science and experience behind every peptide and service from NEP.

Peace of Mind

Not only do we understand the importance of reproducibility in biomarker discovery and proteomics, we also know how to execute consistently time after time.

Ever-Expanding Services

As a partner to proteomics researchers around the world, we know our job will never end. NEP is continually working to expand upon our current service offering.

All of this, plus a company committed above all to the customer experience.