Back

Rat Protocols

Please review our optimized rat protocols below:

Standard Rat Package: $1,300 (90 days)

• Antigen design/Bioinformatics
• 15-mer peptide, 8mg at >85%
• 5mg uncoupled to customer, 2mg coupled to KLH, 1mg for ELISA
• Mass Spec and HPLC trace
• Five immunizations of two rats (Harlan Sprague Dawley)
• ~0.5 mL pre-immune serum per rat
• Day 49 test bleed
• ELISA on test bleed
• Exsanguination (5mL/rat terminal bleed)

Affinity Purified Rat Package: $1,950 (105 days)

• Everything in the Standard Package, plus:
• Extra peptide for affinity purification
• Affinity purification of exsanguinations (10mL antiserum)

Note: Additional rats (up to 10 total) can be added for $350 each with no purification cost added.

Customer Supplied Antigen Standard Rat Package: $900 (70 days)

• Five immunizations of two rats (Harlan Sprague Dawley)
• ~0.5 mL pre-immune serum per rat
• Day 49 test bleed
• ELISA on test bleed
• Exsanguination (5mL/rat terminal bleed)

Customer Supplied Antigen Affinity Purified Rat Package: $1,600 (85 days)

• Everything included in Standard Customer Supplied Package above, plus:
• Affinity purification of 40-50mL antiserum

Material supplied for affinity column generation should be ~2mg and have free sulfhydryl or amine groups for coupling to the affinity matrix.

Immunization and Bleed Schedule:

Day 0 Pre-immune

Day 0 Boost (CFA)

Day 14 Boost (IFA)

Day 28 Boost (IFA)

Day 42 Boost (IFA)

Day 49-50 Test Bleed

Day 56 Boost (IFA)

Day 66 Exsanguination

We only contract with programs that are AAALAC accredited, OLAW assured, and are USDA licensed to ensure all proper care is taken and research animals are treated appropriately. Please inquire for our registration numbers if you are required to have them for compliance purposes.