Custom Polyclonal Antibodies
Not only do we make premium quality peptides for all research applications, but our custom polyclonal antibodies have been recognized as being second-to-none. New England Peptide’s scientists have over a decade of practical experience in all aspects of polyclonal antibody development and production. From this we have gained a perspective into how crucial quality is for materials that are used for research. We therefore pledge to give you the best customer service and technical support possible - from the initial design of your antigen to the end of your project and beyond - to ensure that you, the end user, reap the benefits of that quality when you choose us as your research partner.
Track Record
Our effort and due diligence in achieving the best results for you - unparalleled in the industry - has proven to be worth it. NEP’s track record in polyclonal antibody production speaks for itself:
• >99% success rate by ELISA
• Average ELISA titer of >1:1,100,000
ELISA Guarantee
We guarantee a minimum of a 1:15,000 ELISA titer on any sequence that we design (for at least one of the two rabbits), which in our experience should also translate to a discernable signal in a variety of tests, including westerns and staining applications. No matter the results, we will work closely with you to determine if any adjustments can be made to the protocol that may yield more success. NEP reserves the right to discontinue any project if we feel there is not a legitimate chance for it to attain a 1:15,000 titer.
Expertise from Experience
Our protocols are optimized to get you the best polyclonal antibodies in the best time periods. All the pertinent details, including recommended peptide properties, animal management and total project duration are given below. Alternatively, we welcome any opportunity to assist you in the design of special projects.
Commercial Package: $1,210
• Antigen design/Bioinformatics
• 15-mer peptide, 10mg at >50%
• Mass Spec and HPLC trace
• Three immunizations of two rabbits (New Zealand White)
• 5mL pre-immune serum per rabbit
• Two production bleeds (40-50mL antiserum) per rabbit
• ELISA on 1st production bleed
Standard Package: $1,300
• Antigen design/Bioinformatics
• 15-mer peptide, 10mg at >85%
• 5mg uncoupled to customer, 4mg coupled to KLH, 1mg for ELISA
• Mass Spec and HPLC trace
• Three immunizations of two rabbits (New Zealand White - SPF)
• 5mL pre-immune serum per rabbit
• Two production bleeds (40-50mL antiserum) per rabbit
• ELISA on 1st production bleed
Affinity Purified Antibody Package: Add $650
Everything included in packages above, plus:
• Extra peptide for affinity column
• Affinity purification of 40-50mL antiserum
Immunization and Bleed Schedule
|
Day 0 |
Prebleed, initial boost (CFA) |
|
Day 14 |
Boost (IFA) |
|
Day 28 |
Boost (IFA) |
|
Day 35 |
Bleed #1 ELISA |
|
Day 40 |
Bleed #2 |
Additional Rabbit Protocols
We can accommodate nearly any need regarding polyclonal antibody production. If you have a recombinant protein or peptide already made, send it to us for antibody generation! Please view our additional rabbit protocols for a description of these services and others we routinely use.
Post-Translational Modification Antibodies
We specialize in designing and producing modification state specific antibodies, including phospho and cleavage site-specific, plus several others (mutation specific, acetylated and methylated lysine, nitrosylated tyrosine, etc.). Just click the link or write tech@newenglandpeptide.com to see what we recommend!
Multiple Species Available
If you are interested in something other than rabbit antibodies, we also routinely produce polyclonal antibodies in: Chicken, Goat (or Sheep), Guinea Pig, and Rat. Many other species - from mouse to monkey - are available upon request.
Accreditations
NEP contracts with programs that are AAALAC accredited, OLAW assured, and USDA licensed to ensure all proper care is taken and research animals are treated appropriately. Please inquire for our registration numbers if you are required to have them for compliance purposes.
Product Flexibility
We also recognize that different customers have different needs, whether it be additional material over what is included in our set packages, different formulations of purified antibody, or specialization of the antibodies for use in various assays. That is why we offer multiple other options for additional work, including conjugation of your antibody to biotin or colloidal gold. Please see our options page for details.
Still Need More Information?
There is a lot about polyclonal antibody development that we haven’t yet been able to address. As an additional resource, we have the answers to several common questions on our FAQ page. If your particular question is not there, or you simply want to get in touch with someone that is friendly, knowledgable, and eager to help, call to talk to us right away or email to have us respond within one working day. We look forward to making your research work for you!
